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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, H, M, Po, R, Rb | ELISA, FC, IP, WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1683 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Ankyrin Antibody, clone Ank016 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC. |
| Key Applications |
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| Application Notes | Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals. ELISA: 1:1000 Immunoprecipitation: 1:100 Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10 RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular. Flow cytometry: 1:500 Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended. RIA: 1:5000 Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified human erythroid cells ankyrin |
| Clone | Ank016 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues). |
| Isotype | IgG2a |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P16157 # Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu17 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils. SIZE: 1881 amino acids; 206265 Da SUBUNIT: Interacts with a number of integral membrane proteins and cytoskeletal proteins. Binds SPTB/spectrin (beta chain) through a 70 AA N-terminal region of the 62 kDa domain, and the C-terminal of SLC4A1/erythrocyte membrane protein band 3 through the ankyrin repeat region. Also interacts with TTN/titin. Isoform Mu17 interacts with OBSCN isoform 3/obscurin. SUBCELLULAR LOCATION: Isoform Er1: Cytoplasm. Note=Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane. & Isoform Mu17: Membrane. Cytoplasm, myofibril, sarcomere, M-band. Note=Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells. & Isoform Mu18: Sarcoplasmic reticulum (Probable). & Isoform Mu19: Sarcoplasmic reticulum (Probable). & Isoform Mu20: Sarcoplasmic reticulum (Probable). TISSUE SPECIFICITY: Isoform Mu17, isoform Mu18,isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain. DOMAIN: SwissProt: P16157 The 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3. & The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters. PTM: Regulated by phosphorylation. & Palmitoylated.DISEASE:SwissProt: P16157 # Defects in ANK1 are a cause of hereditary spherocytosis (HS) [MIM:182900]. Inheritance can be autosomal dominant or recessive. SIMILARITY: Contains 23 ANK repeats. & Contains 1 death domain. & Contains 1 ZU5 domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
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| Material Size | 50 µg |