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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, R, Rb | IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1612 |
| Replaces | 04-588 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cytokeratin Epithelial Antibody, clone AE1 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in 0.05M Borate Buffered Saline, 0.15M NaCl, 0.09% Sodium Azide, pH8.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Cytokeratin Epithelial Antibody, clone AE1 is validated for use in IH(P) for the detection of Cytokeratin Epithelial. |
| Key Applications |
|
| Application Notes | Immunohistochemistry on unfixed frozen sections and formalin-fixed paraffin embedded tissues. Antigen retrieval is recommended. HEIR or protease treatment can be used. Western Blotting: AE1 recognizes LMW cytokeratins from the acidic subfamily including: Clone AE1 recognises the 56.5, 50, 50, 48 and 40kD human cytokeratins of the acidic subfamily. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Human epidermal keratin |
| Clone | AE1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes most of the acidic (Type I) keratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. MAB1612 produces heterogeneous staining within a given epithelium; the detailed staining pattern varies depending on the differentiated and/or growth state of individual cells. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05787 # Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. SIZE: 483 amino acids; 53704 Da SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. DISEASE:SwissProt: P05787 # Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600]. SIMILARITY: SwissProt: P05787 ## Belongs to the intermediate filament family. MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 500 µg |