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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-881 |
| Description | Anti-acetyl Cortactin Antibody |
| Alternate Names |
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| Background Information | Src substrate cortactin (UniProt: Q14247; also known as Amplaxin, Oncogene EMS1) is encoded by the CTTN (also known as EMS) gene (Gene ID: 2017) in human. Cortactin is a peripheral membrane protein that serves as a substrate for Src tyrosine kinase and plays a role in regulating cell motility. It is shown to interact with F-actin to promote its polymerization and branching and contributes to the organization of the actin cytoskeleton and cell shape. It also plays a role in the formation of lamellipodia and in cell migration. It contains an N-terminal acidic domain, six and a half tandem repeats of a unique 37-amino acid sequence, and a C-terminal Src homology (SH3) domain. The repeat regions are shown to be essential for F-actin binding. Cortactin is activated via phosphorylation by upstream kinases in response to extracellular stimuli. In mice, Src kinase primarily phosphorylates cortactin at tyrosines 421, 466, and 482, which leads its interaction with F-actin and Arp2/3. It can also be phosphorylated by ERK on serine 405 and 418 that results in recruitment of additional actin nucleation-promoting factors (NPFs), most importantly N-WASP, to the Arp2/3/ complex. Deacetylation of cortactin by HDAC6 is reported to block its translocation to the cell periphery and block its association with F-actin and impairs cell motility. Overexpression of cortactin has been reported in many human tumors and its expression correlates well with the metastatic potential of breast cancer cells and hepatocellular carcinomas. (Ref.: Zhang, X., et al. (2007). Mol. Cell. 27(2); 197-213; Chuma, M., et al. (2004). J. Hepatol. 41(4); 629-636; Li, Y., et al. (2001). Cancer Res. 61(18); 6906-6911; Meiler, E. et al. (2012). PLoS One. 7(3); e33662). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-acetyl Cortactin, Cat. No. 09-881, is a rabbit polyclonal antibody that detects Acetyl-Cortactin and is tested for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide surrounding acetylated lysine site of human cortactin. |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects acetylated form of Cortactin. |
| Species Reactivity |
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| Species Reactivity Note | Human and mouse. Predicted to react with rat and drosophila based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq] |
| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. SUBUNIT STRUCTURE: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 By similarity. Interacts with PLXDC2. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton By similarity. Cell projection › lamellipodium By similarity. Cell projection › ruffle By similarity. Note: Associated with membrane ruffles and lamellipodia By similarity. SEQUENCE SIMILARITIES: Contains 7 cortactin repeats. Contains 1 SH3 domain. |
| Molecular Weight | ~80 kDa observed; 61.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in lysate from HeLa cells treated with Trichostatin A and Nicotinamide. Western Blotting Analysis: A 1:400 dilution of this antibody detected immunoprecipitated Acetyl-Cortactin in lysate from HeLa cells treated with 13.2 mM Trichostatin A and 20 mM Nicotinamide (12 h), but not in lysate from untreated cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |