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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | WB, IHC, IP, IF | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10521 |
| Description | Anti-Neuropilin-1 Antibody |
| Alternate Names |
|
| Background Information | Neuropilin-1 is a type 1 membrane protein with three distinct functions. First, it can mediate cell adhesion via a heterophilic molecular interaction. Second, in neuronal cells, neuropilin-1 binds the class 3 semaphorins, which are neuronal chemorepellents, and plays a role in the directional guidance of axons. It is expected to form complexes with the plexinA subfamily members and mediate the semaphorin-elicited inhibitory signals into neurons. Third, in endothelial cells, neuropilin-1 binds a potent endothelial cell mitogen, vascular endothelial growth factor (VEGF)165, and regulates vessel formation. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit serum in buffer containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Neuropilin-1 Antibody is validated for use in WB, IH, IP, IF for the detection of Neuropilin-1. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | This antibody is generated from rabbits immunized with a His-tagged partial recombinant rat Neuropilin-1. |
| Epitope | Unknown |
| Host | Rabbit |
| Specificity | This antibody recognizes Neuropilin-1. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with mouse and rat. Human-94% homology |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis (By similarity). SUBUNIT: Heterodimer with NRP2 (Probable). Binds PLXNB1 (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Found in the embryonic nervous system. SIMILARITY: Belongs to the neuropilin family. Contains 2 CUB domains. Contains 2 F5/8 type domains. Contains 1 MAM domain. |
| Molecular Weight | 130 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in PC12 cell lysate. Western Blot Analysis: : 1:1,000 dilution of this antibody detected Neuropilin 1 on 10 µg of PC12 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |