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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, FC, IP | Ht | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-963 |
| Description | Anti-CTLA4 (CD152) Antibody, clone 9H10 |
| Alternate Names |
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| Background Information | CTLA4, Cytotoxic T lymphocyte antigen 4, also known as CD152, belongs to the family of immunoglobins. It is widely expressed on activated T lymphocytes and is involved in the maintenance of immune tolerance. CTLA4 is a homolog for CD28. They are able to bind to B7-1 and B7-2; however, CTLA4 is able to bind to both with a higher affinity. Defects in CTLA4 have been linked to Rheumatoid arthritis, Addisons disease, Graves disease and many other autoimmune diseases. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified hamster monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4) with 150 mM NaCl and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CTLA4 (CD152) Antibody, clone 9H10 is an antibody against CTLA4 (CD152) for use in WB, FC & IP. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: Representative images from a previous lot of primary antibody that was biotinylated. Results with 04-963 are expected to be similar if used directly with an anti-Syrian hamster conjugated antibody, however because of the low expression of CTLA4 Fab fragmented goat anti-Syrian hamster biotin followed SA-fluorophore is recommended for best results. FACS analysis of ConA (24 hours) stimulated BALB/C splenocytes for surface expression of CTLA-4 (CD152). Cells were stained with 10 ug of biotin-labeled hamster anti-CTLA-4 ,clone 9H10 (open histogram) or with PE-Sav secondary antibody alone (histogram, Figure B). Note the distinct signal difference between the control (dark control histogram) and test (clear histogram). Immunoprecipitation: Clone 9H10 is widely used for immunoprecipitaiton of CTLA-4; recommended lysis buffers include 1% NP40; 0.5% triton X-100 with proteinase inhibitors; 1-5 µg of 9H10 is added to 300-500 µL of whole cell lysate or cell membrane protein preparations (250-500 mg/mL totall protein) and incubated 3-6 hours at 4°C, followed by capture with protein G beads following standard immunoprecipitation methods (Zhang, Y., et al., 1997). |
| Biological Information | |
|---|---|
| Immunogen | Mouse CTLA-4/human IgG1 fusion protein corresponding to residues surrounding CTLA4. |
| Clone | 9H10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Hamster |
| Specificity | This antibody recognizes CTLA4. Antibody 9H10 is specific for CTLA-4 by four criteria: (a) Reactivity against CTLA-4 Ig but not CD4 lg; (b) the ability to block CTLA-4 Ig binding to B7 transfectants; (c) the ability to stain activated T cells but not freshly isolated T cells; and (d) the ability to stain a CTLA-4 transfectant but not control transfectants (Krummel, M.F., et al., 1995). |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Possibly involved in T-cell activation. Binds to B7-1 (CD80) and B7-2 (CD86). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Widely expressed with highest levels in lymphoid tissues. INVOLVEMENT IN DISEASE: Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variation in CTLA4 may be a cause of susceptibility to Graves disease (GRD) [MIM:275000]. GRD is an autoimmune disorder causing overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to insulin-dependent diabetes mellitus type 12 (IDDM12) [MIM:601388]. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. Celiac disease [MIM:212750] is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. Genetic variations in CTLA4 are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation. SEQUENCE SIMILARITIES: Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| Molecular Weight | ~30 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse spleen lysate. Western Blot Analysis: 1 µg/mL of a biotinylated version of this antibody detected CTLA4 in 10 µg of mouse spleen lysate. THIS ANTIBODY IS NO BIOTINYLATED. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable at 2-8°C for 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |