Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | IP, WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-825 |
| Replaces | 05-825 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
|
| Presentation | Cultured supernantant in 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PDGFRβ Antibody, clone 4A56 is validated for use in IP, WB for the detection of PDGFRβ. |
| Key Applications |
|
| Application Notes | Westerm Blot Analysis: A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells. Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PDGFRβ from 500 μg of 3T3/A31 RIPA lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 710-718 of human PDGF Receptor β. An N-terminus cysteine was added to facilitate conjugation. This sequence corresponds to amino acids 709-717 of mouse PDGF Receptor β. |
| Clone | 4A56 |
| Host | Rabbit |
| Specificity | PDGFRβ, does not cross-react with EGF receptor |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P09619 # Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. SIZE: 1106 amino acids; 123968 Da SUBUNIT: Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DISEASE: SwissProt: P09619 # A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML). & A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia. & A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1. & A chromosomal aberration involving PDGFRB is a cause in many instances of chronic myeloproliferative disorder with eosinophilia (MPE) [MIM:131440]. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein. & A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP- PDGFRB fusion protein. SIMILARITY: SwissProt: P09619 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. |
| Molecular Weight | 190kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |