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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-822 |
| Replaces | 05-822 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal |
| Alternate Names |
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| Background Information | Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Cultured supernantant in 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C). |
| Key Applications |
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| Application Notes | Western Blot Analysis: A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility |
| Clone | 3L74 |
| Host | Rabbit |
| Specificity | Recognizes Rho (-A, -B, -C) |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Broad species cross-reactivity expected based on sequence homology |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P61586 # Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. COFACTOR: Magnesium. SIZE: 193 amino acids; 21768 Da SUBUNIT: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. SUBCELLULAR LOCATION: Membrane; Lipid-anchor. DOMAIN: SwissProt: P61586 The basic-rich region is essential for yopT recognition and cleavage. PTM: Substrate for botulinum ADP-ribosyltransferase. & Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage. SIMILARITY: Belongs to the small GTPase superfamily. Rho family. |
| Molecular Weight | Mr 22kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |