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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-742 |
| Replaces | 05-742 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-SHP-1/2 Antibody, clone NL213, rabbit monoclonal |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Cultured supernantant in 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-SHP-1/2 Antibody, clone NL213 is validated for use in IP, WB for the detection of SHP-1/2. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria |
| Clone | NL213 |
| Host | Rabbit |
| Specificity | Recognizes SHP-1 greater than SHP-2, Mr ~65 kDa |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human and mouse. Others not tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P29350 # Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. SIZE: 595 amino acids; 67561 Da SUBUNIT: Monomer. Interacts with MTUS1 (By similarity). Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, CD300LF and CD84. SUBCELLULAR LOCATION: Cytoplasm. Nucleus (By similarity). Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). TISSUE SPECIFICITY: Isoform 1 is expressed in hematopoietic cells while isoform 2 is expressed in non-hematopoietic cells. PTM: Phosphorylated on serine and tyrosine residues. SIMILARITY: SwissProt: P29350 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. & Contains 2 SH2 domains. & Contains 1 tyrosine-protein phosphatase domain. |
| Molecular Weight | ~65kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot in RIPA lysates from human A431 carcinoma cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |