Sigma-Aldrich Lisorb Rp18 5mym Hi-Rt125

Product Name

LiChrosorb^®RP-18 (5 µm) Hibar^®RT 125-4, suitable for HPLC

Material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

Product Line

LiChrosorb^®

Feature

endcapped: no

Parameter

400 bar pressure
60 °C max. temp.

Technique(S)

HPLC: suitable
mass spectrometry (MS): suitable (Low)

Column L × I.D.

12.5 cm × 4 mm

Matrix

fully porous particle

Related Categories

HPLC Columns

General Description

HPLC columnLiChrosorb^®RP-18 is a reliable irregular shaped silica gel carriers with reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar 125-4 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.

Application

• Simultaneous determination of ampicillin and metampicillin in biological fluids using high-performance liquid chromatography with column switching.: This research introduced an HPLC method with column switching to simultaneously determine ampicillin and metampicillin in biological fluids. The use of LiChrosorb RP-8 (5 micro m) in this method enhanced the separation efficiency, crucial for therapeutic drug monitoring (Lee et al., 1995) (Lee et al., 1995).

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (Toluene): ≥ 55000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 2.0Hibar is a registered trademark of Merck KGaA, Darmstadt, GermanyLiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany