Sigma-Aldrich Nh2 F254s 25 Hptlc Plates

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

200 μm

Plate L × W

10 cm × 10 cm

Particle Size

5-6 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glass plates 10 x 10 cmOur NH2, CN and DIOL -modified silica sorbents are less polar then conventional silica phases, making them ideal for separating hydrophilic or charged substances. Our moderately polar cyano- and diol-modified silica plates can be used for both normal phase and reversed phase systems. An alternative to PEI cellulose, amino-modified NH2 plates provide weak basic ion exchange characteristics with special selectivity for charged compounds.

Application

Glass HPTLC Silica gel 60 NH₂F₂₅₄s plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.

Linkage

Replaces: 15647-5; 15647

Analysis Note

• Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
• Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
• d 50 (laser diffraction, size distribution): 5 - 7 µm
• Layer thickness: 150 - 200 µm
• Deviation of layer thickness per plate: ≤ 35 µm

• Chromatographic testing:
• hRf-values: Amino acid
• - Dansyl-DL-Tryptophan: 40 - 55
• - Dansyl-DL- Phenylalanine: 50 - 65
• - Dansyl-L-Isoleucine: 56 -75

• Color test:
• -a-Naphtholbenzein: 21 - 40
• - Rhodamin B: 51 - 69
• - indophenol blue: 70 - 80