Sigma-Aldrich Lichrospher Si 60 F254s 25

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

200 μm

Plate L × W

20 cm × 10 cm

Particle Size

5-6 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glass plates 20 x 10 cmUnique HPTLC Lichrospher^®plates are the first thin layer chromatography plates based on spherical silica particles. They offer the ultimate in thin layer chromatography performance and speed enabling high throughput analysis of complex samples.

Linkage

Replaces: 115445-1; 115445

Analysis Note

Layer thickness: 170 - 190 µm
Deviation of layer thickness per plate: ≤ 20 µm
Chromatographic testings:
A) Colour test
hRf-values
- Violett 1, colour test, lipophile:
5 - 15
- Nitroechtblue, colour test, lipophile: 20 - 30
- Sico- Fettblue, colour test, lipophile: 50 - 60
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- D-Aldosterone, steroid test:
5 - 15
- Cortisone, steroid test: 20 - 30
- Progesterone, steroid test: 45 - 57
separation number
- steroid test:
≥ 10.0
Typical value determined on a conditioned plate
Eluent A) Toluene (20% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany