Sigma-Aldrich Rp-8 F254s 25 Hptlc Plates

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

HPTLC: suitable

Layer Thickness

200 μm

Plate L × W

10 cm × 10 cm

Particle Size

5-6 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

General Description

25 Glass plates 10 x 10 cm

Linkage

Replaces: 13725-5; 13725

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
48 - 60
B) Steroid test
- methyltestosterone, steroid test: 31 - 51
- Reichstein S, steroid test: 59 - 79
- hydrocortisone, steroid test: 72 - 92
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)