Sigma-Aldrich Silica Gel 60 F254 25 Tlc

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

250 μm

Plate L × W

20 cm × 20 cm

Particle Size

10-12 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glass plates 20 x 20 cmThese plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples. Our concentrating zone plates are based on different adsorption properties of two adsorbents. The first is a large pore concentrating adsorbent where the samples are applied; the second is a selective layer for separation. Regardless of the spots’ shape, size, or position, the sample always concentrates as a narrow band where the two adsorbents overlap and where the separation starts. These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples.

Application

Glass TLC plate, silica gel coated with flourescent indicator F254. The silica gel TLC plates come in a quantity of 25 size 20x20 cm for thin layer chromatography applications.

Linkage

Replaces: 11798-7; 11798

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 10 - 12 µm
Layer thickness: 170 - 250 µm
Deviation of layer thickness per plate: ≤ 50 µm
layer thickness concentrating zone: 90 - 130 µm
Fluctuation in the thickness of the concentration zone: ≤ 35 µm
Chromatographic testing:
colour test
hRf-values
- bleu vif organol, colour test, lipophile:
15 - 21
- Ceres Black G, colour test, lipophile: 36 - 42
- ceres Violet brn, colour test, lipophile: 51 - 58
Typical value determined on a conditioned plate
Eluent: toluene (45% rel. humidity)