Sigma-Aldrich Cellulose 25 Hptlc Plates

Material

cellulose matrix
glass support

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator: no

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

60-110 μm

Plate L × W

10 cm × 10 cm

Storage Temp.

2-30°C

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Thin Layer Chromatography (TLC)

General Description

25 Glass plates 10 x 10 cmCellulose plates are used to analyze polar substances. An organic sorbent, cellulose is perfect for separating hydrophilic substances by partition chromatography. Typical applications include the analysis of amino acids, carbohydrates, and phosphates as well as nucleic acid and nucleic acid derivatives. We offer cellulose plates in two grades: TLC for conventional and HPTLC for demanding, high-performance separations.

Application

• Fast assessment of planar chromatographic layers quality using pulse thermovision method:Focuses on the quality control of cellulose HPTLC plates, ensuring the reliability of chromatographic processes in various research and industrial applications (Suszyński et al., 2014).



• High-performance thin-layer chromatographic-densitometric determination of secoisolariciresinol diglucoside in flaxseed:Utilizes cellulose HPTLC plates for precise quantification of bioactive compounds in natural products, serving the needs of the nutraceutical and pharmaceutical industries (Coran et al., 2004).

Linkage

Replaces: 5787-5; 5787

Analysis Note

Layer thickness: 60 - 110 µm
Deviation of layer thickness per plate: ≤ 30 µm
Aminoacid test
hRf-values
- L-serine, amino acid test:
20 - 30
- L-threonine, amino acid test: 30 - 40
- L-alanine, amino acid test: 35 - 45
- L-methionine, amino acid test: 55 - 65
- L-phenylalanine, amino acid test: 60 - 70
- L-leucine, amino acid test: 70 - 80
Typical value determined on a plate
Eluent: 1-Butanol/ acetone/ acetic acid/ water (35/35/7/23 v/v/v/v) twice developed