Sigma-Aldrich Cellulose F 50 Tlc Plates

Material

cellulose matrix
glass support

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 50 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

80-120 μm

Plate L × W

20 cm × 10 cm

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

50 Glass plates 10 x 20 cmOur cellulose plates are used to analyze polar substances. An organic sorbent, cellulose is perfect for separating hydrophilic substances by partition chromatography. Typical applications include the analysis of amino acids, carbohydrates, and phosphates as well as nucleic acid and nucleic acid derivatives. We offers cellulose plates in two grades: TLC for conventional and HPTLC for demanding, high-performance separations.

Linkage

Replaces: 5728-6; 5728

Analysis Note

Layer thickness: 80 - 120 µm
Deviation of layer thickness per plate: ≤ 30 µm
Aminoacid test
hRf-values
- L-serine, amino acid test:
20 - 30
- L-threonine, amino acid test: 29 - 36
- L-alanine, amino acid test: 30 - 40
- L-methionine, amino acid test: 49 - 60
- L-phenylalanine, amino acid test: 59 - 70
- L-leucine, amino acid test: 66 - 76
Typical value determined on a plate
Eluent: 1-Butanol/ acetone/ acetic acid/ water (35/35/7/23 v/v/v/v) twice developed