Sigma-Aldrich Cellulose F 25 Tlc Plates

Material

cellulose matrix
glass support

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

80-120 μm

Plate L × W

20 cm × 20 cm

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glass plates 20 x 20 cmOur cellulose plates are used to analyze polar substances. An organic sorbent, cellulose is perfect for separating hydrophilic substances by partition chromatography. Typical applications include the analysis of amino acids, carbohydrates, and phosphates as well as nucleic acid and nucleic acid derivatives. We offer cellulose plates in two grades: TLC for conventional and HPTLC for demanding, high-performance separations.

Application

• HPTLC/DESI-MS imaging of tryptic protein digests separated in two dimensions.: This study by Pasilis et al. explores the use of High-Performance Thin-Layer Chromatography (HPTLC) combined with Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for imaging tryptic protein digests. The application of cellulose F TLC plates was crucial in separating protein fragments, providing high-resolution and accurate mass spectrometry analysis. This method showcases the versatility and efficiency of cellulose F TLC plates in advanced proteomics research (Pasilis et al., 2008).

Linkage

Replaces: 5718-7; 5718

Analysis Note

Layer thickness: 80 - 120 µm
Deviation of layer thickness per plate: ≤ 30 µm
Aminoacid test
hRf-values
- L-serine, amino acid test:
20 - 30
- L-threonine, amino acid test: 29 - 36
- L-alanine, amino acid test: 30 - 40
- L-methionine, amino acid test: 49 - 60
- L-phenylalanine, amino acid test: 59 - 70
- L-leucine, amino acid test: 66 - 76
Typical value determined on a plate
Eluent: 1-Butanol/ acetone/ acetic acid/ water (35/35/7/23 v/v/v/v) twice developed