Sigma-Aldrich Aluminium Oxide 60 F254 25

Material

aluminum oxide matrix
glass support

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

250 μm

Plate L × W

20 cm × 20 cm

Particle Size

10-12 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glass plates 20 x 20 cmAluminiumoxide plates are used to analyze basic and neutral compounds at different pH levels. Depending on the pH range in question, professionals can choose between two kinds of aluminum oxide plates. Under aqueous conditions, basic aluminum oxide plates are best for separating basic compounds, while neutral plates are ideal for separating neutral compounds. Available with or without a fluorescence indicator, our TLC aluminum plates handle a wide variety of applications thanks to neutral and basic aluminum oxides with 60 Å and 150 Å pore sizes.

Application

• Surface-enhanced resonance Raman spectroscopy as an identification tool in column liquid chromatography:This research utilizes TLC plates in conjunction with surface-enhanced resonance Raman spectroscopy to refine the identification processes in liquid chromatography. This method is particularly useful for chemists and material scientists who require precise analytical techniques for complex sample matrices in pharmaceutical and environmental labs, thus meeting the needs of principal investigators and lab technicians in various sectors (Seifar RM et al., 2000).

Linkage

Replaces: 5713-7; 5713

Analysis Note

Layer thickness: 210 - 270 µm
Deviation of layer thickness per plate: ≤ 45 µm
d 10 (laser diffraction, size distribution): 3 - 5 µm
d 50 (laser diffraction, size distribution): 8 - 12 µm
d 90 (laser diffraction, size distribution): 18 - 25 µm
chromatographic testing:
alkaloid test
hRf-values
- brucine, alkaloid test:
50 - 65
- papaverine, alkaloid test: 65 - 85
Typical value determined on a plate:
Eluent: Dichloromethane/ methanol (98/2 v/v)

Storage Class

11 - Combustible Solids