Sigma-Aldrich Rp-18 F254s 20 Tlc Alumini

Material

aluminum support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 20 sheets

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

200 μm

Sheet L × W

20 cm × 20 cm

Particle Size

10-12 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

20 Aluminium sheets 20 x 20 cmRP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Application

Aluminum TLC plates, RP-18 modified silica gel coated with flourescent indicator F254s. The RP-18 modified silica gel TLC plates come in a quantity of 20 size 20x20 cm for thin layer chromatography applications.

Linkage

Replaces: 5559-7; 5559

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.7 - 11.7 µm
Layer thickness: 160 - 200 µm
Deviation of layer thickness per plate: ≤ 30 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
32 - 39
B) Steroid test
- methyltestosterone, steroid test: 14 - 22
- Reichstein S, steroid test: 26 - 36
- hydrocortisone, steroid test: 36 - 46
Typical value determined on an activated sheet (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) aceton/ water (60/40 v/v)