| Description | |
|---|---|
| Catalogue Number | 14-526 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Met Protein, active, 10 0 |
| Overview | N-terminal His6-tagged recombinant human Met residues 974-end |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged recombinant human Met residues 974-end, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. |
| Gene Symbol |
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| Protein Target | Met |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | TK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. SIZE: 1390 amino acids; 155527 Da SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior. SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain. |
| Molecular Weight | 50kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of KKKSPGEYVNIEFG |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | 2 vials at 5μg each Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-526M when ordering the 250μg size. |