| Description | |
|---|---|
| Catalogue Number | 14-484 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PKCα Protein, active, 10 µg |
| Overview | Recombinant, full-length human PKCα, containing N-terminal His6 tag PKC Lipid activator (Catalog # 20-133) is required for use. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed by baculovirus in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. |
| Gene Symbol |
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| Protein Target | PKCα |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity). SIZE: 672 amino acids; 76764 Da SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine. SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. |
| Molecular Weight | 78kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of Histone H1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size. |