| Description | |
|---|---|
| Catalogue Number | 14-452 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PDK1 Protein, active, 10 µg |
| Overview | N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. |
| Product Information | |
|---|---|
| Presentation | 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Human PDK1 expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Gene Symbol |
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| Protein Target | PDK1 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. SIZE: 556 amino acids; 63152 Da SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. TISSUE SPECIFICITY: Appears to be expressed ubiquitously. PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain. |
| Molecular Weight | 59kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by phosphorylation of PDKtide |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size. |