| Description | |
|---|---|
| Catalogue Number | 12-459 |
| Brand Family | Upstate |
| Trade Name |
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| Description | IKK Substrate Peptide |
| Product Information | |
|---|---|
| Presentation | Lyophilized powder |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Biological Information | |
|---|---|
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. |
| Gene Symbol |
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| Protein Target | IKKα |
| Target Sub-Family | Misc |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O15111 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. Phosphorylates Ser-10 of histone H3 at NF-kappa-B- regulated promoters during inflammatory responses triggered by cytokines. SIZE: 745 amino acids; 84654 Da SUBUNIT: Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP (By similarity). Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. A weak interaction with TRAF2 cannot be excluded. Part of a complex composed of NCOA2, NCOA3, IKKB, IKBKG and CREBBP. Interacts with NALP2. May interact with MAVS/IPS1. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus. TISSUE SPECIFICITY: Widely expressed. PTM: Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated. SIMILARITY: SwissProt: O15111 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated as a substrate for IKKα (Catalog #14-461) and IKKβ (Catalog #14-485) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 1 mg |