Sigma-Aldrich Leishmans Eosine Methylen

Quality Level

400

Form

solid

Ph

5-6 (0.01% in methanol: water (1:1))

Bulk Density

700 kg/m³

Εmax

650-850 at 522-525 nm in methanol at 0.0005%
800-1100 at 623-627 nm in methanol at 0.0005%

Application(S)

diagnostic assay manufacturing
hematology
histology

Storage Temp.

2-30°C

Related Categories

Dry Dyes & Certified Stains

Hematology Stains

General Description

The dry mixture of the Leishman′s eosin methylene blue dye for microscopy, is used for the self-preparation of the Leishman′s staining solution. The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue and May-Grünwald′s eosin methylene blue, it is used for staining of blood and bone marrow smears and clinical cytological specimens. The dye is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Identity (UV/VIS-Spectrum): passes test
pH-value (0,01 %; methanol 50 %): 5 - 6
Absorption maximum λ 1 (Methanol): 623 - 627 nm
Absorption maximum λ 2 (Methanol): 522 - 525 nm
Spec. Absorptivity A 1%/1cm
- λ1_{m a x}; 0.0005 %; methanol; calculated on dried substance:
800 - 1100
- λ2_{m a x}; 0.0005 %; methanol; calculated on dried substance: 650 - 850
TLC-Test: passes test
Loss on drying (105 °C): ≤ 8 %
Suitability for microscopy: passes test

Pictograms

GHS07

Signalword

Warning

Hcodes

H302 - H317 - H319

Pcodes

P261 - P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 3