LI-COR IRDye® 800cw Goat Anti-Mouse Igg1-Specific Secondary Antibody, 0.5 mg

Immunogen

Mouse IgG₁ paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG₁ covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG₁. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG_2a, IgG_2b, IgG₃, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ Assay applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

Applications

Highly recommended for:

• Western Blot
• In-Cell Western Assay
• On-Cell Western Assay
• Protein Array
• Immunohistochemistry
• Small Animal Imaging
• Microscopy
• 2D Gel Detection
• Tissue Section Imaging
• Virus Titration Assay

Formulation

IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

RRID

• P/N 926-32350: RRID AB_2782997

Example Data