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LI-COR IRDye® 680rd Donkey Anti-Goat Igg Secondary Antibody, 0.1 mg
List Price
$121.97
Your Price
$121.97
LI-COR Anti-Goat IgG Antibody, 0.1 mg Net Content, 1 mg/mL, Polyclonal, Conjugate: IRDye 680RD, Donkey Host Species, Solid Form, 4 deg C - LIC (Additional S&H or Hazmat Fees May Apply)
NETA PART:
LIC-925-68074
MFG.PART:
925-68074
UNSPSC:
12352203
Manufacturer:
LI-COR BioScience
Immunogen
Goat IgG
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and with sheep IgG. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with human, mouse, rabbit, rat, chicken. guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ Assay applications.
Applications
Highly recommended for:
- Western Blot
- In-Cell Western Assay
- On-Cell Western Assay
- Protein Array
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
Formulation
IRDye 680RD Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey® Western blot detection | 1:20,000 | 1:5,000 - 1:25,000 |
| In-Cell Western Assay detection | 1:800 | 1:200 - 1:1,200 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
Note: Do not use this secondary antibody in combination with any secondary antibody whose host is goat (for example, IRDye 800CW Goat anti-Rabbit) for two-color Western blot detection.
RRID
- P/N 925-68074: RRID AB_2650427
- P/N 926-68074: RRID AB_10956736
| SKU | LIC-925-68074 |
|---|---|
| Featured | No |
| Supplier Part Number | 925-68074 |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | LI-COR BioScience |
| CountryOfOrigin | United States |
| ProductLine | LIC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |
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