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LI-COR IRDye® 680lt Donkey Anti-Chicken Secondary Antibody, 0.1 mg
List Price
$121.97
Your Price
$121.97
LI-COR IRDye 680LT Donkey anti-Chicken IgG (H + L), Highly Cross-Adsorbed, 0.1 mg - LIC (Additional S&H or Hazmat Fees May Apply)
NETA PART:
LIC-925-68028
MFG.PART:
925-68028
UNSPSC:
12352203
Manufacturer:
LI-COR BioScience
Immunogen
Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.
Purity and Specificity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
Applications
Recommended for:
- Western Blot
- Protein Array
- Immunohistochemistry
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
Not Recommended for:
- Small Animal Imaging
- In-Cell Western Assay
- On-Cell Western Assay
Formulation
IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey Western blot detection | 1:20,000 | 1:10,000 - 1:50,000 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 - 0.02%) and Tween® 20 (final concentration of 0.1 - 0.2%) must be added during the detection incubation step to avoid non-specific background staining.
RRID
- P/N 925-68028: RRID AB_2814923
- P/N 926-68028: RRID AB_10707008
Example Data
| SKU | LIC-925-68028 |
|---|---|
| Featured | No |
| Supplier Part Number | 925-68028 |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | LI-COR BioScience |
| CountryOfOrigin | United States |
| ProductLine | LIC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |
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