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LI-COR IRDye® 680lt Donkey Anti-Goat Igg Secondary Antibody, 0.1 mg
List Price
$121.97
Your Price
$121.97
LI-COR IRDye 680LT Donkey anti-Goat IgG (H + L), Highly Cross-Adsorbed, 0.1 mg - LIC (Additional S&H or Hazmat Fees May Apply)
NETA PART:
LIC-925-68024
MFG.PART:
925-68024
UNSPSC:
12352203
Manufacturer:
LI-COR BioScience
Immunogen
Goat IgG
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using goat IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with human, mouse, rabbit, rat, chicken. guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
Applications
Recommended for:
- Western Blot
- Protein Array
- Immunohistochemistry
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
Not Recommended for:
- Small Animal Imaging
- In-Cell Western Assay
- On-Cell Western Assay
Note: Do not use this secondary antibody in combination with any secondary antibody whose host is goat (for example, IRDye 800CW Goat anti-Rabbit) for two-color Western blot detection.
Formulation
IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey Western blot detection | 1:20,000 | 1:10,000 - 1:50,000 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 - 0.02%) and Tween® 20 (final concentration of 0.1 - 0.2%) must be added during the detection incubation step to avoid non-specific background staining.
RRID
- P/N 925-68024: RRID AB_2814908
- P/N 926-68024: RRID AB_10706168
Example Data
| SKU | LIC-925-68024 |
|---|---|
| Featured | No |
| Supplier Part Number | 925-68024 |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | LI-COR BioScience |
| CountryOfOrigin | United States |
| ProductLine | LIC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |