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GenScript Gencrispr Lbcas12a Nuclease; 100ug
List Price
$189.00
Your Price
$189.00
GenScript Gencrispr™ Lbcas12a Nuclease,100ug - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-Z03753-100
MFG.PART:
Z03753-100
UNSPSC:
12352204
Manufacturer:
GenScript USA Inc
Overview
| Description | GenCRISPR™ LbCas12a Nuclease is an RNA-guided DNA endonuclease from Lachnospiraceae bacterium. Cas12a (previously known as Cpf1) belongs to the Class 2 Type V CRISPR/Cas system. Different from CRISPR/Cas9 system, the ribonucleoprotein (RNP) complex of CRISPR/Cas12a system is formed by Cas12a and crRNA, it recognizes a T-rich protospacer adjacent motif (PAM) and results in a staggered DNA double-strand break (DSB). After the specific cleavage, Cas12a can also activate collateral cleavage activity towards adjacent non-specific ssDNA sequences. Hence, Cas12a nuclease is a good alternative for Cas9 in certain target DNA editing, and provides a novel strategy for DNA detection. |
Applications
| crRNA-dependent DNA cleavage |
Properties
| Source | Recombinant Cas12a with a C-terminal NLS expressed by E.coli |
| Species | Lachnospiraceae bacterium |
| Tag | C-terminal 6× His Tag |
| Apparent Molecular Weight | 146 kDa, on SDS-PAGE under non-reducing conditions |
| Concentration | 4 mg/ml |
| Storage Buffer | 10 mM Tris-HCl, 300 mM NaCl, 0.5 mM DTT, 50% glycerol, pH 7.4. |
| Storage & Stability | Store at -20 °C for up to 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| Accession# | 6KL9_A |
Quality Control Specifications
| Appearance | Clear, colorless liquid |
| Purity | ≥ 90% by SDS-PAGE |
| Concentration by A280 | 4(± 10%) mg/ml |
| Bioactivity (in vitro) | ≥ 90% |
| Residual DNase | Undetectable |
| Residual RNase | Undetectable |
| Endotoxin Level | < 0.1 EU/μg |
Background
| References | 1. Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system." Cell 163.3 (2015): 759-771. 2. Ledford, Heidi. "Alternative CRISPR system could improve genome editing." Nature News 526.7571 (2015): 17. 3. Chen, Janice S., et al. "CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity." Science 360.6387 (2018): 436-439. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03753-100 |
|---|---|
| Featured | No |
| Supplier Part Number | Z03753-100 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer Name | GenScript USA Inc |
| Temperature | Store at -20 °C for up to 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| CountryOfOrigin | China |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |