GenScript Anti-Ha Ip Resin; 1mL Resin (Total 2 mL)

List Price $400.00 Your Price $400.00
GenScript Anti-Ha Ip Resin - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-L00777-1
MFG.PART: L00777-1
UNSPSC: 41106510
Manufacturer: GenScript USA Inc
Description

Principle
Add the sample containing the HA-tagged protein to the Anti-HA IP Resin and allow the protein bind to the Resin during a short time incubation. Then the HA-tagged protein can be eluted off the beads, or used directly for pull-down of target protein.


Material Supplied
GenScript Anti-HA IP Resin is an agarose beads coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244). The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).
Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at -20°C.Do not store the gel at freezing temperatures in the absence of glycerol. 
Store the Column: Wash the column three times with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml of TBS/A and store at 2-8°C without draining.
Additional Buffers Needed
Equilibration buffer:  50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.

Overview
Product Information Characteristics of Anti-HA IP Resin
Resin Volume1 ml settled resin (2 ml 50% slurry)
LigandAnti-HA monoclonal antibody against HA tag
Number of HA-tagged protein per ligand2
Binding capacityApproximately 1 mg HA-tagged protein (~20kDa) per ml settled resin
Storage solution1X PBS containing 50 % glycerol
Storage and stabilityStore at -20 °C for up to 12 months.
Resin reuseThe resin can be recycled for at least 3 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


More Information
SKU GSCRPT-L00777-1
Featured No
Supplier Part Number L00777-1
UM EA
UNSPSC 41106510
Manufacturer Name GenScript USA Inc
ProductLine GSCRPT
Qty 1
MinOrderQty 1
Weight 7.000000
Lead Time 7
Hazardous N
Energy Star No
Green No
ACT Ecolabel No
Controlled N
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