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GenScript High Affinity Ni-Charged Resin Ff; 100mL Resin (Total 200 mL)
List Price
$875.00
Your Price
$875.00
GenScript High Affinity Ni-Charged Resin Ff - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-L00666-100
MFG.PART:
L00666-100
UNSPSC:
41106510
Manufacturer:
GenScript USA Inc
Description
GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins.
Sample: 5 mg/mL (histidine)6-tagged pure proteins (Mr 43 000) in binding buffer (capacity at 10% Column volume: 1 mL Retention time: 3min Flow rate: 0.333 mL/min Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4 Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4 Note: Dynamic binding capacity is protein-dependent. Reagents Compatible with High Affinity Ni-Charged Resin FF
‡ The strong chelator EDTA has been used successfully in some cases at 1 mM. Generally, chelating agents should be used with caution (and only in the sample, not in buffers). Any metal ion stripping may be counteracted by addition of a small excess of MgCl2 before centrifugation/filtration of the sample. Note that stripping effects may vary with applied sample volume. |
For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.
| SKU | GSCRPT-L00666-100 |
|---|---|
| Featured | No |
| Supplier Part Number | L00666-100 |
| UM | EA |
| UNSPSC | 41106510 |
| Manufacturer Name | GenScript USA Inc |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |
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