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Sigma-Aldrich Nadh Approx. 98% 1 G
List Price
$175.35
Your Price
$175.35
Sigma-Aldrich Nadh Approx. 98% 1 G - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART:
SIAL-10128023001
MFG.PART:
10128023001
UNSPSC:
12352204
Manufacturer:
Sigma-Aldrich
Quality Level
100
Assay
98%
Form
amorphous powder
Mol Wt
(NADH-Na2: Mr= 709.4
NADH: Mr= 665.4)
Packaging
pkg of 1 g
Manufacturer/Tradename
Roche
Storage Condition
protect from light
Λmax
260 and 340 nm
Shipped In
wet ice
Smiles String
O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
Related Categories
Roche® Life Science Products
Application
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.Biochem/Physiol Actions
NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Packaging
Packaged by solid weight.Quality
Purity:98% β-NADH-Na2based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A260nm-A340nm: <2.40Organic solvents:<4% (GC)
Specifications
Absorbance coefficients:14.3 x 103l x mol-1x cm-1at 260 nm, 6.18 x 103l x mol-1x cm-1at 334 nm (Hg), 6.3 x 103l x mol-1x cm-1at 340 nm, 3.4 x 103l x mol-1x cm-1at 365 nm (Hg)Formula:C21H27N7O14P2Na2
pH value in water:7.5 ± 0.5 (100 mg/ml)
Physical Form
Amorphous powderPreparation Note
Activator:Mg2+(5 to 10 mM)Storage conditions (working solution):10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C
Storage And Stability
Store at 2 to 8 °C. (Store dry!)Other Notes
For life science research only. Not for use in diagnostic procedures.Storage Class
11 - Combustible Solids
Wgk_Germany
WGK 1
Flash_Point_F
Not applicable
Flash_Point_C
Not applicable
| SKU | SIAL-10128023001 |
|---|---|
| Featured | No |
| Supplier Part Number | 10128023001 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer Name | Sigma-Aldrich |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |