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EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line
Overview
JK1 is an immortalized CD34+SMA+ cell line derived from spontaneous transformation of C57/BL6 mouse testicular stromal cells. JK1 cells are able to support the long-term culture of a variety of primitive cells including multi-potent adult spermatogonial-derived stem cells, pluripotent murine embyronic stem cells and embyronic germ cells derived from primordial germ cells. Adult spermatogonial stem and progenitor cells (SPCs) cultured on JK1 feeder cells express key features of germ line stem cells including expression of PLZF, DAZL, and GCNA1. JK1 feeder cells has maintained its capacity for promoting stem cell self-renewal even after serial passaging for over one year.
Alternate Names
JK1 Feeder Cells
Applications
Application
Immortalized Mouse Feeder Cell Line
Key Applications
Cell Culture
Stem Cell Culture
Application Notes
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
Fibroblast Cells
Product Usage Statements
Quality Assurance
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services. • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services • Cells are negative for mycoplasma contamination.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
