Cell Signaling Hsl Antibody

Name: Cell Signaling Hsl Antibody
SKU: CSIG-4107T
Price: 132.31 USD

List Price $132.31 Your Price $132.31

Cell Signaling Hsl Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4107T

MFG.PART: 4107T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	81, 83
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	HSL Antibody detects endogenous levels of total HSL protein.
Species Reactivity	Human, Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human HSL. Antibodies are purified by peptide affinity chromatography.
Background	HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).

Specifications