| Description | |
|---|---|
| Catalogue Number | MABE1135-100UG |
| Description | Anti-TRF2 Antibody, clone T2JKM3 |
| Alternate Names |
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| Background Information | Telomeric repeat-binding factor 2 (UniProt: Q15554; also known as TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein, TERF2) is encoded by the TERF2 (also known as TRBF2, TRF2) gene (Gene ID: 7014) in human. TERF2 is homodimeric protein that is ubiquitous in its expression. Higher expression levels are reported in spleen, thymus, prostate, uterus, testis, peripheral blood leukocytes, colon, and small intestine. Two soforms of TERF2 are reported that are produced by alternative splicing.TERF2 binds to telomeric double-stranded 5-TTAGGG-3 repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. It is also required to recruit a number of factors and enzymes required for telomere protection, including the sheltering complex, TERF2IP/RAP1 and DCLRE1B/Apollo. TEFR2 colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase. It is phosphorylated upon DNA damage and the phosphorylated TERF2 is not bound to telomeric DNA, and rapidly localizes to damage sites. Inhibition of TRF2 causes apoptosis in several mammalian cell types and its over-expression is observed in a variety of human cancers. |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-TRF2, clone T2JKM3, Cat. No. MABE1135, is a highly specific mouse monoclonal antibody that targets Telomeric repeat-binding factor 2 (TRF2) and has been tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
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| Application Notes | Immunocytochemistry Analysis: A representative lot detected TRF2 in IMR90 primary human fibroblasts. Western Blotting Analysis: A 1:200 dilution from a representative lot detected TRF2 in HT1080 cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | Tagged full length recombinant human Telomeric repeat-binding factor 2. |
| Clone | T2JKM3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone T2JKM3 detects Telomeric repeat-binding factor 2 in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~60 kDa observed; 59.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HT1080 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected TRF2 in HT1080 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |