Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1064-25UG |
| Description | Anti-HsSAS-6 Antibody, clone 91.390.21 |
| Alternate Names |
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| Background Information | Spindle assembly abnormal protein 6 homolog (UniProt: Q6UVJ0; also known as HsSAS-6) is encoded by the SASS6 (also known as SAS6) gene (Gene ID: 163786) in human. HsSAS-6 is a central scaffolding component of the centrioles that ensure their 9-fold symmetry. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. It is required for centrosome biogenesis and duplication and is essential for both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multi-ciliated cells. It contains a 35 nM long coiled-coil domain that mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring. Over-expression of SASS6 gene results in excess foci-bearing centriolar markers. Mutations in SASS6 are known to cause microcephaly 14 that is characterized by small head circumference, markedly reduced brain weight and disproportionately small cerebral cortex. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect SASS6 using this mouse monoclonal Anti-HsSAS-6, clone 91.390.21, Cat. No. MABE1064-25UG, validated for use in Immunofluorescence, Immunoprecipitation and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected HsSAS-6 in 10 µg of HeLa cell lysate. Western Blotting Analysis: A representative lot detected HsSAS-6 in RNAi in U2OS cell lysates (Courtesy of Professor Dr. Erich Nigg, Ph.D; Biozentrum, University of Basel, Switzerland). Immunofluorescence Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunofluorescence (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland). Immunofluorescence Analysis: A representative lot detected HsSAS-6 in Immunofluorescence applications (Kleylein-Sohn, J. et. al. (2007). Dev Cell. 13(2):190-202 ; Sonnen, K..,et. al. (2012). Biol Open 1(10):965-76; Arquint, C., et al. (2012). J Cell Sci. 125(Pt 5):1342-52 ). Immunoprecipitation Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunoprecipitation (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to 254 amino acids from the C-terminal half of human Spindle assembly abnormal protein 6 homolog (HsSAS-6). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 91.390.21 detects HsSAS-6 protein in human cells. It targets an epitope with in 254 amino acids in the C-terminal half. |
| Isotype | IgG2b |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in U20S cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected HsSAS-6 in 10 µg of U2OS cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |