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Millipore Anti-Beclin-1 Antibody, Clone 9a1.1 Detects Level Of Beclin-1 & Has Been Published & Validated For Use In Western Blotting, Ihc(P), Flow Cytometry. - Mill (Additional S&H or Hazmat Fees May Apply)
The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinsons and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).
Product Information
Format
Purified
Control
Raji cell lysate
Presentation
Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Beclin-1 Antibody, clone 9A1.1 detects level of Beclin-1 & has been published & validated for use in Western Blotting, IHC(P), Flow Cytometry.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Flow Cytometry
Application Notes
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Beclin-1 in human colorectal adenocarcinoma tissue.
Flow Cytometry Analysis: 1 µg from a representative lot detected Beclin-1 in HeLa cells.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Beclin-1.
Clone
9A1.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_003757
Gene Symbol
BECN1
GT197
Purification Method
Protein G Purified
UniProt Number
Q14457
Molecular Weight
~54 kDa kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Beclin-1 in 10 µg of Raji cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
