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Millipore Anti-Blue, Clone 2d2-F11, Is A Mouse Monoclonal Antibody That Detects Prestained Precision Plus Protein Marker Proteins And Has Been Tested For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-Blue antibody, Clone 2D2-F11, is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. The use of this antibody allows for visualization of marker proteins along with the protein of interest on the same blot. Anti-Blue antibody offers high specificity and does not interfere with the detection of other antibodies, In addition, it does not display reactivity with other cellular proteins, unstained marker proteins, or with proteins stained with different dyes.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins and has been tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected BLUE in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).
Biological Information
Immunogen
Prestained Precision Plus Protein marker proteins.
Clone
2D2-F11
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins.
Isotype
IgG1κ
Species Reactivity
All
Species Reactivity Note
All.
Antibody Type
Monoclonal Antibody
Purification Method
Protein G purified
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Pre-Stained Protein Standard.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected BLUE-stained marker protein standards.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
