Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), WB, Proximity Ligation Assay | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1608-25UG |
| Description | Anti-Mucin-16 (CA-125) Antibody, clone 5E11 |
| Alternate Names |
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| Background Information | Mucin-16 (UniProt: Q8WXI7; also known as MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125) is encoded by the MUC16 (also known as CA125) gene (Gene ID: 94025) in human. Mucin-16 is a heavily O-glycosylated protein that can be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus, which induces proteolytic cleavage and liberation of extracellular domain. I provides protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Mucin-16 is composed of three domains: a Serine, Threonine-rich N-terminal domain, a repeated domain containing between 12 and 60 partially conserved tandem repeats of 156 amino acids and a C-terminal transmembrane domain with a short cytoplasmic tail. Its expression is up-regulated in ovarian cancer cells and may serve as a marker for ovarian cancer. Mucin-16 is shown to binds to mesothelin (MSLN) and this binding mediates heterotypic cell adhesion, which may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Mucin-16 using this mouse monoclonal Anti-Mucin-16 (CA-125), clone 5E11 Antibody, Cat. No. MABC1608-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting, Proximity Ligation Assay. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (C ourtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal). Proximity Ligation Assay Analysis: A representative lot detected Mucin-16 in Proximity Ligation Assay applications (Ricardo, S., et. al. (2016). Virchows Arch. 468(6):715-22; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12). Immunohistochemistry Analysis: A representative lot detected Mucin-16 in Immunohistochemistry applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12). Immunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (C ourtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal). Western Blotting Analysis: A representative lot detected Mucin-16 in Western Blotting applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to 264 amino acids from the C-terminal half of human Mucin-16. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5E11 detects Mucin-16 in human ovarian cancer tissue. It targets an epitope within 264 amino acids from the C-terminal half. |
| Isotype | IgG1 |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human ovarian cancer and human uterine tissues. Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution of this antibody detected Mucin-16 in human ovarian cancer and human uterine tissues. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |