Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, FC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1606-25UG |
| Description | Anti-Caspase-8 Antibody, clone 3B10 |
| Alternate Names |
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| Background Information | Caspase-8 (UniProt: O89110; also known as EC: 3.4.22.61, CASP-8, CASP8, MCH5, FLICE, MACH) is encoded by the Casp8 gene (Gene ID: 12370) in murine species. Caspase-8 is a cysteine protease that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. In its inactive form it associates with FADD by interactions between their DED motifs and within the FAS-FADD-Caspase 8 complex caspase 8 undergoes self-cleavage to convert to the active form. Activated caspase 8 is subsequently released to the cytosol and it cleaves downstream effector caspases 3, 4, 6, 7, 9 and 10. Caspase 8 is expressed in wide variety of tissues and its highest expression has been reported in spleen, thymus, lung, liver, and kidney. Low levels of expression are observed in heart, brain, testis, and skeletal muscle. Caspase-8 is produced with propetides (aa 1-218 and 377-387) that are cleaved under appropriate signals to produce a p20 (aa 219-376) and a p10 (aa 388-480) subunit. Active caspase 8 is a heterotetramer that consists of two p20 and two p10 subunits. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG1 in PBS with 0.02% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Caspase-8, clone 3B10, Cat. No. MABC1606, is a rat monoclonal antibody that detects Caspase-8 and has been tested for use in Flow Cytometry and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 1 µg from a representative lot detected Caspase-8 in one million mouse embryonic fibroblasts (MEF-1). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant p20 subunit of Mouse Caspase-8. |
| Clone | 3B10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | This rat monoclonal antibody detects Caspase-8 in murine species. It targets an epitope within the p20 subunit. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~55 kDa observed; 55.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse embryonic fibroblast (MEF-1) cell lysates. Western Blotting Analysis: 1 µg/mL of this antibody detected Caspase-8 in mouse embryonic fibroblast (MEF-1) cell lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |