Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1220 |
| Description | Anti-RGS2 Antibody, clone 9D9.1 |
| Alternate Names |
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| Background Information | Regulator of G-protein signaling 2 (UniProt: P41220; also known as RGS2, Cell growth-inhibiting gene 31 protein, G0/G1 switch regulatory protein 8) is encoded by the RGS2 (also known as G0S8, GIG31) gene (Gene ID: 5997) in human. RGS2 is shown to regulate G protein-coupled receptor signaling cascades. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. It also plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. RGS2 is reported to play a role in regulating the constriction and relaxation of vascular smooth muscle and also has a protective effect against myocardial hypertrophy and atrial arrhythmias. It inhibits beta-adrenergic receptor-induced cardiomyocyte hypertrophy. Overexpression of RGS2 reduces the activity of isoproterenol-induced ERK1/2 and prevents AKT activation. Four isoforms of RGS2 have been reported that are produced by alternative splicing. (Ref.: Nunn, C et al. (2010). Cell Signal 22(8); 1231-1239). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-RGS2, clone 9D9.1, Cat. No. MABC1220, is a mosue monoclonal antibody that detects RGS2 in human and rat cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected RGS2 in 10 µg of HL-60 cell lysate. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RGS2 in human cerebral cortex, human pancreas, human lymphoma, and rat testis tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 82 amino acids from the N-terminal region of human Regulator of G-protein signaling 2 (RGS2). |
| Clone | 9D9.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 9D9.1 specifically detects Regulator of G-protein signaling 2 (RGS2) in human and rat cells. It targets an epitope within 82 amino acids from the N-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Rat.. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~25 kDa observed; 24.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected RGS2 in 10 µg of Raji cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |