Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, Ch, R, H | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1190-25UG |
| Description | Anti-Bin1 Antibody, clone 2F11 |
| Alternate Names |
|
| Background Information | Myc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. Bin1 is a ubiquitous protein that displays highest expression levels in the brain and muscle. Eleven isoforms of Bin1 have been described that are generated by alternate splicing. Isoform IIA is reported to be expressed only in the brain where it is concentrated n axon initial segments and nodes of Ranvier. It contains one BAR domain (aa 29-276) and one SH3 domain (aa 520-592). Bin1 interacts with the functionally critical Myc box regions at the N- terminus of the MYC oncoprotein and inhibits MYC-induced malignant cell transformation. Hence, it is considered as a tumor suppressor. Mutations in BIN1 gene are known to cause centronuclear myopathy that is characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Bin1 using this mouse monoclonal Anti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates. Immunohistochemistry Analysis: A representative lot detected Bin1 in Immunohistochemistry applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42). Western Blotting Analysis: A representative lot detected Bin1 in Western Blotting applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42; Muller, A.J., et. al. (2003). Mol Cell Biol. 23(12):4295-306; Chang, M.Y., et. al. (2007). Cancer Res. 67(1):100-7). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to the BAR domain (aa 29-276) from the N-terminal half of human Myc box-dependent-interacting protein 1 (Bin1). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2F11 recognizes an epitope within the N-terminal BAR domain of Bin 1. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Chicken, Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in human cerebellum and human skeletal muscle tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Bin1 in human cerebellum and human skeletal muscle tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |