Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ELISA | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1167-25UL |
| Description | Anti-P53DINP1 Antibody, clone F8 |
| Alternate Names |
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| Background Information | Tumor protein p53-inducible nuclear protein 1 (UniProt: Q96A56; also known as Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1) is encoded by the TP53INP1 (also known as P53DINP1, SIP) gene (GeneID: 94241) in human. p53DINP1 is a ubiquitously expressed protein with anti-proliferative and pro-apoptotic properties and is involved in cell stress response. It shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions. It acts as a dual regulator of transcription and autophagy. p53DINP1 acts as a positive regulator of autophagy and in response to cellular stress or upon activation of autophagy, it relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C to regulate autophagy. p53DINP1 also acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. It has both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. p53DINP1 is reported to positively regulate p53/TP53 and p73/TP73 and stimulate their capacity to induce apoptosis. In response to double-strand DNA breaks, it promotes p53/TP53 phosphorylation on Ser46 and subsequent apoptosis. It is shown to acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect using this rat monoclonal Anti-P53DINP1, clone F8 Antibody, Cat. No. MABC1167-25UL, validated for use in Western Blotting and ELISA. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected P53DINP1 in 10 µg of human brain tumor, breast cancer, lymphoma, and pancreatic cancer tissue lysates. ELISA Analysis: A representative lot detected P53DINP1 in Elisa applications (Saadi, H., et. al. (2013). Results Immunol. 3:51-6). Western Blotting Analysis: A representative lot detected P53DINP1 in Western Blotting applications (Al Saati, T., et. al. (2013). Am J Pathol. 182(6):1996-2004; Peuget, S., et. al. (2014). Cell Death Differ. 21(7):1107-18; Saadi, H., et. al. (2013). Results Immunol. 3:51-6; Seillier, M., et. al. (2012). Cell Death Differ. 19(9):1525-35). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full length recombinant human Tumor protein p53-inducible nuclear protein 1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone F8 detects P53DINP1 (TP53INP1) protein in various human cancer cells. |
| Isotype | IgG2a |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human colon tissue lysate. Western Blotting Analysis: A 1:62.5 dilution of this antibody detected P53DINP1 in 10 µg of human colon tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |