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| Description | |
|---|---|
| Catalogue Number | MAB3418-25UG |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MAP-2, clone AP20 |
| Alternate Names |
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| Background Information | MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB. |
| Key Applications |
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| Application Notes | Western Blot: 60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels. Immunohistochemistry: 5 µg/mL (4% PFA fixed frozen sections). Immunohistochemistry: 5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde. Optimal working concentrations must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Bovine brain microtubule protein. |
| Clone | AP20 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | MAB3418 binds specifically to MAP-2. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Rat and mouse. Expected to cross-react with human, cow, chicken, quail, and Xenopus. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SIZE: 1827 amino acids; 199539 Da PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats. |
| Molecular Weight | 230 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Rat Brain lysate. Western Blot Analysis: 1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |