Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IHC, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1946-I-25UL |
| Description | Anti-Nidogen 1 Antibody, clone ELM1 |
| Alternate Names |
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| Background Information | Nidogen-1 (UniProt: P10493; also known as NID-1, Entactin) is encoded by the Nid1 (also known as Ent) gene (Gene ID: 18073) ins murine species. Nidogen-1 is a highly conserved sulfated monomeric glycoprotein that is widely distributed in basement membranes and tightly associates with laminin. It is post-translationally N- and O-glycosylated. It is synthesized with a 28 aa signal peptide that is subsequently cleaved to produce the mature form. Nidogen -1 contains six EGF-like domains, four LDL-receptor class B repeats, one NIDO domain, one nidogen G2 barrel domain, and one thyroglobulin type 1 domain. Nidogen-1 shares about 50% homology with Nidigen-2 and shares similar adhesive properties. There is also a high degree of homology between murine and human Nidogen-1. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rat monoclonal Anti-Nidogen 1, clone ELM1 Antibody, Cat. No. MAB1946-I-25UL is validated for use in Immunohistochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Nidogen-1 in Western Blotting applications (Arends, F., et. al. (2015). PLoS One. 10(2):e0118090). Immunocytochemistry Analysis: A representative lot detected Nidogen-1 in Immunocytochemistry applications (Kick, K., et. al. (2016). Arterioscler Thromb Vasc Biol. 36(12):2346-2357). Immunohistochemistry Analysis: A representative lot detected Nidogen-1 in Immunohistochemistry applications (Chang, Y.T., et. al. (2016). J Clin Invest. 126(2):721-31). |
| Biological Information | |
|---|---|
| Immunogen | Purified protein from basement membranes of mouse Engelbreth-Holm-Swarm (EHS) tumor. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone ELM1 is a rat monoclonal antibody that detects Nidogen-1 in murine species. |
| Isotype | IgG2a |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse kidney. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Nidogen-1 in mouse kidney tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |