Cell Signaling Spar (D9p9t) Rabbit mAb

Name: Cell Signaling Spar (D9p9t) Rabbit mAb
SKU: CSIG-25823S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Spar (D9p9t) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-25823S

MFG.PART: 25823S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	13
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	SPAR (D9P9T) Rabbit mAb recognizes endogenous levels of total SPAR protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SPAR protein.
Background	Small regulatory Polypeptide of Amino Acid Response (SPAR) is a polypeptide, encoded by the lncRNA LINC00961 gene and is localized in late endosomal and lysosomal membranes. SPAR binds to the lysosomal v-ATPase complex in addition to ragulator, RagA and RagC. Upon amino acid stimulation, this supercomplex activates mTORC1 signaling. However, this process is inhibited by the binding of SPAR to the v-ATPase complex, as its formation prevents mTORC1 recruitment to lysosomes. SPAR downregulation and inactivation is an important focus for promoting muscle regeneration following muscle injury. This downregulation and inactivation promotes mTORC1 activation and results in myofiber maturation. In addition to muscle regeneration, SPAR downregulation and inactivation also promotes stem cell proliferation and differentiation.

Specifications