Cell Signaling Doublecortin (A8l1u) Rabbit mAb

Name: Cell Signaling Doublecortin (A8l1u) Rabbit mAb
SKU: CSIG-14802S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Doublecortin (A8l1u) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-14802S

MFG.PART: 14802S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunofluorescence (Frozen): 1:400}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Doublecortin (A8L1U) Rabbit mAb recognizes endogenous levels of total doublecortin protein.
Species Reactivity	Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human doublecortin protein.
Background	Mutations in doublecortin (DCX) cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule-associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5, and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331, and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

Specifications