| Description | |
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| Catalogue Number | SCR020 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Mesenchymal Adipogenesis Kit |
| Overview | Introduction: Excessive weight gain and obesity pose significant health challenges to many industrialized nations. Many metabolic disorders associated with cardiovascular diseases such as diabetes and atherosclerosis are directly linked to the increased production and size of adipose cells. Understanding the molecular mechanism that underlie adipogenesis, the process by which adipose or fat tissue is formed, is thus of critical importance. Elucidation of the steps involved in adipogenesis, including terminal differentiation, have been facilitated in large part by the development of pre-adipocyte cell lines such as the Swiss 3T3-L1 mouse model (Please refer to CHEMICON®s Adipogenesis Assay Kit, Catalog No. ECM950). However using these model systems to study some of the earliest events in cellular differentiation are problematic as these cells are already lineage-restricted and pre-determined to become adipocytes. Stem cell technology, particularly embryonic stem cells and/or mesenchymal stem cells offer attractive alternative sources of adipocytes for tissue culture studies and for the biochemical dissection of the earliest steps involved in adipose cell determination. Mesenchymal stem cells are multipotent progenitor cells that have the capacity to differentiate into several mesenchymal cell lineages, including bone, cartilage and fat. Millipores Mesenchymal Stem Cell Adipogenesis Kit contains reagents that readily differentiate mesenchymal stem cells to an adipogenic lineage as assessed with Oil Red O staining of lipid vacuoles in mature adipocytes. These factors include dexamethasone, IBMX, insulin and indomethacin. Along with Oil Red O staining solution, a hematoxylin solution is provided to counterstain the cell nucleus. Using Millipores Mesenchymal Stem Cell Adipogenesis Kit, we typically obtain > 30% mature adipocytes from the rat bone marrow derived mesenchymal stem cells. Efficiency of adipogenic differentiation may vary, depending upon the quality of the mesenchymal stem cells and if variations to the protocol are introduced. For Research Use Only; not for use in diagnostic procedure. |
| Materials Required but Not Delivered | 1. Human or rat mesenchymal stem cell and cell culture reagents 2. Mesenchymal Stem Cell Expansion Media (DMEM-low glucose, without glutamine, 10% heat-inactivated fetal bovine serum, 2 mM L-Glutamine and 1X solution of penicillin and streptomycin) 3. 24 - well tissue culture plates 4. Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B) 5. AccutaseTM (Catalog No. SCR005) 6. Fixative (e.g. 4% Paraformaldehyde in 1X PBS) 7. Hemacytometer 8. Microscope |
| Product Information | |
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| Components |
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| Quality Level | MQ100 |
| Applications | |
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| Application | Mesenchymal Stem Cell Adipogenesis Kit typically obtains 30% mature adipocytes from the rat bone marrow derived mesenchymal stem cells. |
| Biological Information | |
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| Stem Cell Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Note: Kit components require two different storage temperatures. Dexamethasone Solution, IBMX Solution, Insulin Solution and Indomethacin Solution should be stored at -20°C. Oil Red O and Hematoxylin Solutions should be stored at room temperature. Storage of Oil Red O Solution at -20°C may result in formation of insoluble precipitates and is not recommended. If Oil Red O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter. Precautions: Oil Red O stains skin and clothing. IBMX, Dexamethasone and Indomethacin are irritants and potentially toxic. DMSO is readily absorbed through the skin. Wear a lab coat and gloves when handling these solutions. Isopropanol is flammable. Keep solutions containing isopropanol (Oil Red O Solution) away from open flames. |
| Packaging Information | |
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| Material Size | 1 kit |