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Sigma-Aldrich Expand Hifi Plus Pcr Sys.
List Price
$718.11
Your Price
$718.11
Sigma-Aldrich Expand Hifi Plus Pcr Sys. 500 U - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART:
SIAL-3300226001
MFG.PART:
3300226001
UNSPSC:
41106300
Manufacturer:
Sigma-Aldrich
Usage
sufficient for ≤2,500 reactions (03300234001)
sufficient for 2500 reactions
sufficient for ≤500 reactions (03300226001)
sufficient for 500 reactions
Quality Level
100
Feature
High Fidelity PCR
dNTPs included: no
hotstart: no
Packaging
pkg of 2,500 U (03300234001 [10x250 U])
pkg of 500 U (03300226001 [2x250 U])
Manufacturer/Tradename
Roche
Parameter
72 °C optimum reaction temp.
Technique(S)
PCR: suitable
Input
purified DNA
Storage Temp.
−20°C
Related Categories
PCR Reagents & Kits
Roche® Life Science Products
General Description
The Expand High FidelityPLUSPCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.The Expand High FidelityPLUSPCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUSPCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.
Application
The Expand™High FidelityPLUSPCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.Use Expand High FidelityPLUS PCR System for:
- PCR
- RT-PCR
- Primer extension
- Carryover prevention
Features And Benefits
- Safeguard against cross-contamination.
- Obtain sixfold higher fidelity and higher yields,
- Efficiently label DNA.
Packaging
1 kit containing 4 componentsQuality
Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.Unit Definition
Volume Activity: 5 U/μLOther Notes
For life science research only. Not for use in diagnostic procedures.Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.Expand is a trademark of RocheH412P273 - P501| SKU | SIAL-3300226001 |
|---|---|
| Featured | No |
| Supplier Part Number | 3300226001 |
| UM | EA |
| UNSPSC | 41106300 |
| Manufacturer Name | Sigma-Aldrich |
| Temperature | 0 TO -20 DEGREE C |
| Refrigerated-Frozen | Y |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |