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RPI X-Gal , 250 Milligrams
List Price
$49.23
Your Price
$49.23
RPI X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside], 250 Milligrams - RPI
NETA PART:
RPI-B71800-0.25
MFG.PART:
B71800-0.25
UNSPSC:
12352204
Manufacturer:
RPI
5-Bromo-4-chloro-3-indolyl-β-D-galactoside
7240-90-6
408.6
C14H15BrCINO6
DMSO and DMF
-20°C
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL resuLts in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene resuLts in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
0 lbs
1.25 x 1.75 x 1.25
Research or further manufacturing use only, not for food or drug use.
| SKU | RPI-B71800-0.25 |
|---|---|
| Featured | No |
| Supplier Part Number | B71800-0.25 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer Name | RPI |
| CountryOfOrigin | Italy |
| ProductLine | RPI |
| Qty | 1 |
| MinOrderQty | 1 |
| Width | 1.3 |
| Depth | 1.3 |
| Height | 1.8 |
| Weight | 7.000000 |
| Lead Time | 3 |
| Hazardous | N |
| CAS Number | 7240-90-6 |
| ACT Ecolabel | No |