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Quality Bio DNA Denaturing Solution 1000ml
List Price
$55.65
Your Price
$55.65
Quality Bio DNA Denaturing Solution 1000ml - QB (Additional S&H or Hazmat Fees Will Apply)
NETA PART:
QB-351-013-131
MFG.PART:
351-013-131
UNSPSC:
41116126
Manufacturer:
Quality Biological
Description
DNA Denaturing Solution is useful for denaturing fractionated DNA prior to the transfer from an electrophoresis gel to nitrocellulose or nylon membrane, as well as Northern and Southern blotting.
- Contains: 0.5 M NaOH and 1.5 M NaCl
- Filtered through a 0.22 µm membrane
- DNase tested
Applications
The Denaturing Solution is a strong base, 0.5 M NaOH with 1.5 M NaCl, that denatures double-stranded DNA, so that it can be efficiently transferred to a nylon or nitrocellulose membrane and subsequently hybridized to a labeled probe.
When utilized in conjunction with a depurination solution, treatment with the denaturing solution results in the hydrolysis of the phosphodiester backbone at the sites of depurination. This allows for a more efficient transfer of large DNA in neutral Southern transfers. Following DNA denaturation, the gel must be neutralized by soaking in DNA Neutralization Solution.
Max Shelf Life: 36 months
Storage Condition: 15°C to 30°C
Shipping Condition: ambient temperature
Sterile Filtered
DNase Tested
UNSPSC: 41116126
Harmonization Code: 3822.00.5090
ECCN: EAR99
| SKU | QB-351-013-131 |
|---|---|
| Featured | No |
| Supplier Part Number | 351-013-131 |
| UM | EA |
| UNSPSC | 41116126 |
| Manufacturer Name | Quality Biological |
| MSDS URL | Click here |
| Temperature | RT |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | QB |
| Qty | 1 |
| MinOrderQty | 1 |
| Width | 12 |
| Depth | 9 |
| Height | 6 |
| Weight | 48.000000 |
| Lead Time | 5 |
| Hazardous | N |
| CAS Number | 7647-14-5 |
| Energy Star | No |
| Green | No |
| Controlled | N |